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Journal / Biology / Mukociliäre Epithelorganoide aus Xenopus…
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Biology

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Mukociliäre Epithelorganoide aus Xenopus Embryonal cells
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Mukociliäre Epithelorganoide aus Xenopus Embryonal cells: Generation, Culture und High-Resolution Live Imaging

Article DOI: 10.3791/61604-v • 07:44 min • July 28th, 2020
July 28th, 2020 •
1, 1
1Center for Vascular Research, Institute for Basic Science

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Wir beschreiben ein einfaches Protokoll zur Entwicklung von mukociliären Epithelorganoiden aus tiefen Ektodermzellen, die aus Xenopus laevis Embryonen isoliert sind. Die multipotenten Vorläufer regenerieren epitheliale Kelchenzellvorläufer und ermöglichen eine Live-Tracking der Initiation und Progression der Zellübergänge auf der Oberfläche von Organoiden.

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Biologie Ausgabe 161 Sphäroid Aggregat mesenchymal-epitheliale Übergang Kelchenzelle multiciliated Zelle Gewebemechanik
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