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Analysere det α-Actinin-nettverket i humane iPSC-avledede kardiomyocytter ved hjelp av enkeltmolekyl lokalisering mikroskopi

Article DOI: 10.3791/61605-v • 07:02 min • November 3rd, 2020
November 3rd, 2020 •
1,2, 1,2, 3, 1,2, 1,2
1Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Rostock University Medical Center, 2Faculty of Interdisciplinary Research, Department Life, Light & Matter, University Rostock, 3Department of Cardiology, Rostock University Medical Center

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Dannelsen av et skikkelig sarcomere-nettverk er viktig for modning av iPSC-avledede kardiomyocytter. Vi presenterer en super oppløsningsbasert tilnærming som muliggjør kvantitativ evaluering av den strukturelle modningen av stamcelleavledede kardiomyocytter, for å forbedre kulturforhold som fremmer hjerteutvikling.

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Biologi Utgave 165 humane induserte pluripotente stamceller kardiomyocytter superoppløsning modning sarkomerenettverk fotoaktivert lokalisering mikroskopi
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