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Immunology and Infection

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JoVE Journal Immunology and Infection
Um modelo de imagem neonatal de sepse bacteriana gram-negativa
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Um modelo de imagem neonatal de sepse bacteriana gram-negativa

Article DOI: 10.3791/61609-v • 08:46 min • August 12th, 2020
August 12th, 2020 •
*1, *1,2, 1, 1, 1,2
1Department of Microbiology, Immunology, & Cell Biology, West Virginia University School of Medicine, 2Vaccine Development Center at West Virginia University Health Sciences Center
* These authors contributed equally

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A infecção de camundongos neonatais com bioluminescente E. coli O1:K1:H7 resulta em uma infecção séptica com inflamação pulmonar significativa e patologia pulmonar. Aqui, descrevemos procedimentos para modelar e estudar mais a sepse neonatal utilizando imagens intravitais longitudinais em paralelo com a enumeração de cargas bacterianas sistêmicas, perfil inflamatório e histopatologia pulmonar.

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Imunologia e Infecção Problema 162 bioluminescência imagem intravital E. coli,inoculação subscapular recém-nascidos sepse inflamação citocinas
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