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En halvautomatisk ChIP-Seq-procedure for store epigenetiske studier

Article DOI: 10.3791/61617-v • 08:04 min • August 13th, 2020
August 13th, 2020 •
1, 1, 1, 1, 1, 1
1Division of Vaccine Discovery, La Jolla Institute for Immunology

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Dette papir beskriver en halvautomatisk, mikroskaleret ChIP-Seq protokol af DNA-områder forbundet med en bestemt histone ændring (H3K27ac) ved hjælp af en ChIP væske-handler platform, efterfulgt af bibliotek forberedelse ved hjælp af tagmentation. Proceduren omfatter kontrolvurdering med henblik på kvalitet og kvantitet og kan anvendes til andre histoneændringer eller transskriptionsfaktorer.

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Biologi Udgave 162 ChIP-Seq ChIP væske-handler tagmentation H3K27ac lav input spånering automatiseret
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