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Journal / Biochemistry / En modell membran plattform for rekonstituere…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
En modell membran plattform for rekonstituere mitokondrie membran dynamikk
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En modell membran plattform for rekonstituere mitokondrie membran dynamikk

Article DOI: 10.3791/61620-v • 10:31 min • September 2nd, 2020
September 2nd, 2020 •
1, 1, 2, 1,3
1Department of Molecular Biology, Massachusetts General Hospital, 2Department of Chemistry, University of Akron, 3Department of Genetics, Harvard Medical School

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Mitokondriefusjon er en viktig homeostatisk reaksjon underliggende mitokondriedynamikk. Beskrevet her er et in vitro rekonstitueringssystem for å studere mitokondrie indre membran fusjon som kan løse membran tethering, docking, hemifusjon, og pore åpning. Allsidigheten til denne tilnærmingen i å utforske cellemembransystemer diskuteres.

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Denne måneden i JoVE utgave 163 mitokondrie indre membran Opa1 polymer-bundet lipid bilayer in vitro rekonstituering membran fusjon TIRF mikroskopi
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