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Journal / Neuroscience / Avledning, utvidelse, kryopreservasjon og…
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JoVE Journal Neuroscience
Avledning, utvidelse, kryopreservasjon og karakterisering av mikrovaskulære endotelceller fra humane induserte pluripotente stamceller
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Avledning, utvidelse, kryopreservasjon og karakterisering av mikrovaskulære endotelceller fra humane induserte pluripotente stamceller

Article DOI: 10.3791/61629-v • 08:04 min • November 19th, 2020
November 19th, 2020 •
1,2,3, 1,2,3,4, 1,3,4,5
1Center for Genomic Medicine, Massachusetts General Hospital, 2Department of Psychiatry, Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, 4Department of Psychiatry, Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program, McLean Hospital

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Denne protokollen beskriver en tilpasset metode for å utlede, utvide og kryopreserve hjernen mikrovaskulære endotelceller oppnådd ved differensiere menneskelige induserte pluripotente stamceller, og å studere blod hjernebarriere egenskaper i en ex vivo modell.

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Nevrovitenskap Utgave 165 Human indusert pluripotente stamceller mikrovaskulære endotelceller blodhjernebarriere trans-endotel elektrisk motstand transportøraktivitet mikrovaskulær schizofreni bipolar lidelse passaging ekspansjon kryopreservation
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