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Journal / Neuroscience / Implantation af osmotiske pumper og induktion af…
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Neuroscience
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JoVE Journal Neuroscience
Implantation af osmotiske pumper og induktion af stress for at etablere en symptomatisk, farmakologisk musemodel for DYT/PARK-ATP1A3 Dystoni
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Implantation af osmotiske pumper og induktion af stress for at etablere en symptomatisk, farmakologisk musemodel for DYT/PARK-ATP1A3 Dystoni

Article DOI: 10.3791/61635-v • 10:41 min • September 12th, 2020
September 12th, 2020 •
1, 1, 1, 1
1Department of Neurology, University Hospital of Würzburg

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Vi leverer en protokol til at generere en farmakologisk DYT/PARK-ATP1A3 dystoni musemodel via implantation af kanyler i basale ganglier og lillehjernen forbundet til osmotiske pumper. Vi beskriver induktion af dystoni-lignende bevægelser via anvendelse af en motorisk udfordring og karakterisering af fænotypen via adfærdsmæssige scoringssystemer.

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Neurovidenskab neurovidenskab farmakologisk musemodel osmotiske pumper motorudfordring stress adfærdsmæssig karakterisering bevægelsesforstyrrelser DYT/PARK-ATP1A3 DYT12 dystoni
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