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Journal / Chemistry / Krystallisering af proteiner på chip af…
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JoVE Journal Chemistry
Krystallisering af proteiner på chip af mikrodialyse til In Situ X-ray Diffraktion Studies
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Krystallisering af proteiner på chip af mikrodialyse til In Situ X-ray Diffraktion Studies

Article DOI: 10.3791/61660-v • 12:38 min • April 11th, 2021
April 11th, 2021 •
1, 1,3, 1, 1, 2, 1
1Université Grenoble Alpes, CEA, CNRS, IBS, 2CNRS, Solvay LOF, UMR 5258, Université Bordeaux, 3ELVESYS SAS

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Dette papir beskriver fabrikationsprotokollen for mikrofluidiske chips udviklet til on-chip protein krystallisering med dialysemetoden og in situ X-ray diffraktionseksperimenter. Mikrofabrikationsprocessen gør det muligt at integrere en semipermeable regenereret cellulosedialysemembran med enhver molekylær vægtafskæring mellem to lag af chippen.

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Kemi Problem 170 mikrofluidics on-chip protein krystallisering in situ X-ray diffraktion mikrodialyse krystallisering seriel krystallografi fase diagrammer dialyse chip
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