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JoVE Journal Neuroscience
In Vitro Wedge Slice Forberedelse til efterligne In Vivo Neuronal Circuit Connectivity
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In Vitro Wedge Slice Forberedelse til efterligne In Vivo Neuronal Circuit Connectivity

Article DOI: 10.3791/61664-v • 10:31 min • August 18th, 2020
August 18th, 2020 •
1, 1
1Section on Neuronal Circuitry, National Institute on Deafness and Other Communication Disorders, NIH

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Integration af forskellige synaptiske input til neuroner måles bedst i et præparat, der bevarer alle præ-synaptiske kerner for naturlig timing og kredsløb plasticitet, men hjernen skiver typisk afskære mange forbindelser. Vi udviklede en modificeret hjerneskive til at efterligne in vivo kredsløbsaktivitet, samtidig med at vitro-eksperimenterne opretholdes.

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Neurovidenskab mediale olivocochlear neuroner in vitro skive elektrofysiologi synaptisk integration auditive nerve auditive hjernestamme cochlear kerne hæmmende neurotransmission
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