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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Article DOI: 10.3791/61665-v • 07:43 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1, 1, 3, 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide
* These authors contributed equally

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Summary
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This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in rice.

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Agrobacterium-mediated Genetic Transformation Transgenic Production Male Reproductive Development Rice Explant Callus Production Gene Function Crop Improvement Regeneration Method Video Demonstration Inflorescences Paddy Field Greenhouse Meiosis Stage Leaf Sheath Alcohol Swab Sterile Lab Bench Petri Plate NBD2 Medium Incubate Dark 26 Degrees Celsius Induce Callus Transformation Selective Antibiotics Liquid YEB Medium Orbital Shaker
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