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JoVE Journal Developmental Biology
Isolering af murine spermatogene celler ved hjælp af en Violet-ophidset celle-permeable DNA bindende dye
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Isolering af murine spermatogene celler ved hjælp af en Violet-ophidset celle-permeable DNA bindende dye

Article DOI: 10.3791/61666-v • 08:21 min • January 14th, 2021
January 14th, 2021 •
*1,2, *1,2, 3,4, 1,2, 3,4, 5,6, 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science
* These authors contributed equally

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Her præsenterer vi en enkel og effektiv metode til at isolere levende meiotiske og post-meiotiske kimceller fra voksne muse testes. Ved hjælp af en lav-cytotoksicitet, violet-ophidset DNA bindende farvestof og fluorescens-aktiveret celle sortering, kan man isolere højt beriget spermatogene cellepopulationer for mange downstream applikationer.

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Udviklingsbiologi spermatogenese meiose spermatocytter spermatider fluorescens-aktiveret cellesortering (FACS) DyeCycle Violet (DCV)
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