Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolatie van Murine Spermatogenic Cellen met…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Isolatie van Murine Spermatogenic Cellen met behulp van een Violet-Excited Cell-Permeable DNA Binding Dye
Collection of Germinal Vesicle Oocytes from the Ovaries of CD-1 Mice and Induction of DNA Double-Strand Breaks (Video) | JoVE
Journal (Developmental Biology)
Collection of Germinal Vesicle Oocytes from the Ovaries of CD-1 Mice and Induction of DNA Double-Strand Breaks (Video) | JoVE
4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy
Journal (Developmental Biology)
4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy
 
Click here for the English version

Isolatie van Murine Spermatogenic Cellen met behulp van een Violet-Excited Cell-Permeable DNA Binding Dye

Article DOI: 10.3791/61666-v • 08:21 min • January 14th, 2021
January 14th, 2021 •
*1,2, *1,2, 3,4, 1,2, 3,4, 5,6, 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier presenteren we een eenvoudige en efficiënte methode om levende meiotische en post-meiotische kiemcellen te isoleren van volwassen muispesten. Met behulp van een lage cytotoxiciteit, violet-opgewonden DNA bindende kleurstof en fluorescentie-geactiveerde celsorde, kan men zeer verrijkte spermatogene celpopulaties isoleren voor vele downstream toepassingen.

Tags

Ontwikkelingsbiologie spermatogenese meiose spermatocyten spermatids fluorescentie-geactiveerde celsorling (FACS) DyeCycle Violet (DCV)
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter