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Journal / Developmental Biology / 보라색 흥분 세포 투과성 DNA 결합 염료를 사용하여 뮤린 정자 세포의…
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Developmental Biology

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JoVE Journal Developmental Biology
보라색 흥분 세포 투과성 DNA 결합 염료를 사용하여 뮤린 정자 세포의 격리
Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
Journal (Developmental Biology)
Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
Journal (Developmental Biology)
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
 
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보라색 흥분 세포 투과성 DNA 결합 염료를 사용하여 뮤린 정자 세포의 격리

Article DOI: 10.3791/61666-v • 08:21 min • January 14th, 2021
January 14th, 2021 •
*1,2, *1,2, 3,4, 1,2, 3,4, 5,6, 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science
* These authors contributed equally

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여기서 우리는 성인 마우스 고환에서 살아있는 메이오틱 및 후 메이오성 세균 세포를 격리하는 간단하고 효율적인 방법을 제시합니다. 낮은 세포 독성, 보라색 흥분 DNA 결합 염료 및 형광 활성화 세포 분류를 사용 하 여, 하나는 많은 다운 스트림 응용 프로그램에 대 한 고도로 농축 된 정자 세포 인구를 격리할 수 있습니다.

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발달 생물학 문제 167 정자 발생 meiosis 정자 세포 정자 형광 활성화 세포 선별 (FACS) 염료 자전거 바이올렛 (DCV)
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