Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolering av murine spermatogene celler ved hjelp …
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Isolering av murine spermatogene celler ved hjelp av en violet-spent celle-gjennomtrengelig DNA bindende fargestoff
Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick
Journal (Developmental Biology)
Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
Journal (Developmental Biology)
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
 
Click here for the English version

Isolering av murine spermatogene celler ved hjelp av en violet-spent celle-gjennomtrengelig DNA bindende fargestoff

Article DOI: 10.3791/61666-v • 08:21 min • January 14th, 2021
January 14th, 2021 •
*1,2, *1,2, 3,4, 1,2, 3,4, 5,6, 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her presenterer vi en enkel og effektiv metode for å isolere levende meiotiske og postmeiotiske bakterieceller fra voksne musetestikler. Ved hjelp av en lav cytotoksisitet, fiolett-spent DNA bindende fargestoff og fluorescens-aktivert celle sortering, man kan isolere svært beriket spermatogene cellepopulasjoner for mange nedstrøms applikasjoner.

Tags

Utviklingsbiologi utgave 167 spermatogenese meiosis spermatocytter spermaater fluorescensaktivert cellesortering (FACS) DyeCycle Violet (DCV)
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter