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JoVE Journal Developmental Biology
Isolamento de células spermatogênicas murinas usando um corante de ligação de DNA permeável celular-permeável violeta
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Isolamento de células spermatogênicas murinas usando um corante de ligação de DNA permeável celular-permeável violeta

Article DOI: 10.3791/61666-v • 08:21 min • January 14th, 2021
January 14th, 2021 •
*1,2, *1,2, 3,4, 1,2, 3,4, 5,6, 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science
* These authors contributed equally

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Aqui apresentamos um método simples e eficiente para isolar células germinativas vivas e pós-meiotic de testículos de camundongos adultos. Usando uma baixa citotoxicidade, corante de DNA com proteção violeta e classificação celular ativada por fluorescência, pode-se isolar populações de células espermatogênicas altamente enriquecidas para muitas aplicações a jusante.

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Biologia do Desenvolvimento Questão 167 espermatogênese meiose espermatos espermatóides classificação celular ativada pela fluorescência (FACS) DyeCycle Violet (DCV)
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