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Demonstration af heterologe komplekser dannet af Golgi-Resident Type III membranproteiner ved anvendelse af Split Luciferase Complementation Assay

Article DOI: 10.3791/61669-v • 05:28 min • September 10th, 2020
September 10th, 2020 •
1, 1, 1
1Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw

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Protokollen, der præsenteres her, har til formål at påvise forekomsten af heterologe interaktioner mellem Golgi-hjemmehørende type III-membranproteiner med cytoplasmatisk eksponerede N- og/eller C-termini i levende pattedyrceller ved anvendelse af den seneste variant af det splittede luciferasekomplementeringsassay.

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Biologi udgave 163 split luciferase komplementering assay protein-protein interaktioner PPI'er luminescens bioluminescens heterologe komplekser Golgi-apparater type III membranproteiner nukleotidsukkertransportører NST'er forbigående transfektion
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