JoVE Journal
Cancer Research
Cancer Research
A subscription to JoVE is required to view this content.
膵臓腺癌の腫瘍微小環境を調べるための近位液の分離
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Click here for the English version
Article
DOI:
10.3791/61687-v
•
05:44 min
•
November 5th, 2020
膵臓腺癌の腫瘍微小環境を調べるための近位液の分離
November 5th, 2020
•
Greta Donisi*1, Marialuisa Barbagallo*2, Giovanni Capretti1,3, Gennaro Nappo1,3, Panteleimon G. Takis4,5,6, Alessandro Zerbi1,3, Federica Marchesi2,7, Nina Cortese2
1Section of Pancreatic Surgery, Humanitas Clinical and Research Center-IRCCS, 2Department of Immunology and Inflammation, Humanitas Clinical and Research Center-IRCCS, 3Department of Biomedical Sciences, Humanitas University, 4Giotto Biotech S.R.L., 5Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, 6National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, 7Department of Medical Biotechnology and Translational Medicine, University of Milan
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
膵液は、ヒト膵臓がんのバイオマーカーの貴重な供給源です。ここでは、術中収集手順の方法について説明します。マウスモデルでこの手順を採用するという課題を克服するために、代替サンプルである腫瘍間質液を提案し、ここではその分離のための2つのプロトコルについて説明します。