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JoVE Journal Genetics
In Vivo CRISPR/Cas9-screening om tegelijkertijd de genfunctie in de huid en mondholte van de muis te evalueren
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In Vivo CRISPR/Cas9-screening om tegelijkertijd de genfunctie in de huid en mondholte van de muis te evalueren

Article DOI: 10.3791/61693-v • 07:52 min • November 2nd, 2020
November 2nd, 2020 •
1, 1,2, 1,2, 3, 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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Hier beschrijven we een snelle en directe in vivo CRISPR/Cas9 screeningsmethodologie met behulp van echogeleide in utero embryonale lentivirale injecties om tegelijkertijd functies van verschillende genen in de huid en mondholte van immunocompetente muizen te beoordelen.

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Deze maand in JoVE in vivo CRISPR ultrasound guided micro injection long-tail CRISPR library muismodellen van kanker snel in vivo scherm
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