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Triagem In Vivo CRISPR/Cas9 para avaliar simultaneamente a função genética na pele do rato e na cavidade oral
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Triagem In Vivo CRISPR/Cas9 para avaliar simultaneamente a função genética na pele do rato e na cavidade oral

Article DOI: 10.3791/61693-v • 07:52 min • November 2nd, 2020
November 2nd, 2020 •
1, 1,2, 1,2, 3, 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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Aqui descrevemos uma metodologia rápida e direta de triagem in vivo CRISPR/Cas9 utilizando ultrassom guiado em injeções lentiviras embrionárias uterobrionárias para avaliar simultaneamente funções de vários genes na pele e cavidade oral de camundongos imunocompetrente.

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Este mês no JoVE Edição 165 in vivo CRISPR micro injeção guiada por ultrassom cauda longa biblioteca CRISPR modelos de mouse de câncer tela in vivo rápida
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