Journal
/
Bioengineering
/
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry…
JoVE Journal
Bioengineering
Bioengineering
This content is Open Access.
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
October 29th, 2020
•
1Helmholtz Institute for Pharmaceutical Research Saarland, Department of Microbial Natural Products, Helmholtz Centre for Infection Research and Department of Pharmacy, Saarland University, 2Partner Site Hannover-Braunschweig, German Centre for Infection Research (DZIF)
* These authors contributed equally
Chapters
Summary
The elucidation of the mode-of-action of a novel antibiotic is a challenging task in the drug discovery process. The goal of the method described here is the application of isothermal microcalorimetry using calScreener in antibacterial profiling to provide additional insight into drug-microbe interactions.
Tags
Metabolic Profiling Bactericidal Bacteriostatic Natural Products Isothermal Microcalorimetry Mode Of Action Antibacterial Compounds Real-time Observation Noninvasive Manner Sample Analysis Microbiological Techniques Data Analysis Spectrophotometer Optical Density Colony Forming Units MHB Medium Concentration Antibiotic Concentration Vortexing Inserts Plastic Inserts 48-well Plate Titanium Vials HoldersRelated Videos
PLAYLIST
Biology
-
Research • BiologyQuantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
-
Research • BioengineeringMetabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
-
Research • BiologyProbing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
-
Research • BiologyPlanarian Scrunching as a Quantitative Behavioral Readout for Noxious Stimuli Sensing
-
Research • BiologyQuantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
Chemistry
-
Research • ChemistryExcitonic Hamiltonians for Calculating Optical Absorption Spectra and Optoelectronic Properties of Molecular Aggregates and Solids
-
Research • ChemistryAutomated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
-
Research • ChemistryHyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
-
Research • ChemistryMass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
-
Research • ChemistryA Microwave-Assisted Direct Heteroarylation of Ketones Using Transition Metal Catalysis
Engineering
-
Research • EngineeringTerahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors
-
Research • EngineeringA Random-displacement Measurement by Combining a Magnetic Scale and Two Fiber Bragg Gratings
-
Research • EngineeringFabricating van der Waals Heterostructures with Precise Rotational Alignment
-
Research • EngineeringCutting Procedures, Tensile Testing, and Ageing of Flexible Unidirectional Composite Laminates
-
Research • EngineeringDeveloping High Performance GaP/Si Heterojunction Solar Cells
Clinical Science
-
Research • MedicineLung CT Segmentation to Identify Consolidations and Ground Glass Areas for Quantitative Assesment of SARS-CoV Pneumonia
-
Research • MedicineEvaluation of Capnography Sampling Line Compatibility and Accuracy when Used with a Portable Capnography Monitor
-
Research • MedicineAssessment of Dependence in Activities of Daily Living Among Older Patients in an Acute Care Unit
-
Research • MedicineInduction of Accelerated Atherosclerosis in Mice: The "Wire-Injury" Model
-
Research • MedicineAn In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices