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急性小鼠脑切片研究自发海马网络活动
Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity

急性小鼠脑切片研究自发海马网络活动

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8,972 Views

07:58 min

August 28, 2020

DOI:

07:58 min
August 28, 2020

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Summary

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此协议描述了从表现出自发锐波波纹活动的小鼠身上制作水平海马-内皮层 (HEC) 切片的准备工作。切片在简化的接口夹腔中孵育,并在水下条件下用快速流动的人工脑脊液进行录音,以促进组织氧合和网络水平活动的自发出现。

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