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JoVE Journal Neuroscience
Akut Musehjerne udskæring for at undersøge spontan hippocampal netværksaktivitet
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Akut Musehjerne udskæring for at undersøge spontan hippocampal netværksaktivitet

Article DOI: 10.3791/61704-v • 07:58 min • August 28th, 2020
August 28th, 2020 •
1
1Department of Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University

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Denne protokol beskriver forberedelsen af vandrette hippocampal-entorhinal cortex (HEC) skiver fra mus, der udviser spontan skarpbølge krusningsaktivitet. Skiver inkuberes i et forenklet grænsefladeholderkammer, og optagelser udføres under neddykkede forhold med hurtigtflydende kunstig cerebrospinalvæske for at fremme iltning af væv og spontan fremkomst af aktivitet på netværksniveau.

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Neurovidenskab Problem 162 hippocampus mus skive elektrofysiologi skarpbølge krusning spontan aktivitet hippocampal-entorhinal cortex netværk interface kammer
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