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Journal / Biochemistry / Isolering af Histone fra Sorghum Leaf Tissue for…
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Biochemistry

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JoVE Journal Biochemistry
Isolering af Histone fra Sorghum Leaf Tissue for Top Down Massespektrometri Profilering af potentielle epigenetiske markører
PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
Journal (Biochemistry)
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Journal (Biochemistry)
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Isolering af Histone fra Sorghum Leaf Tissue for Top Down Massespektrometri Profilering af potentielle epigenetiske markører

Article DOI: 10.3791/61707-v • 07:10 min • March 4th, 2021
March 4th, 2021 •
1, 1, 2, 2, 2, 1, 1, 1, 3, 3, 3, 4,5, 6, 6, 1, 6, 1, 1
1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute, Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center, University of California Agriculture and Natural Resources, 4West Side Research and Extension Center, University of California, 5Department of Plant Sciences, University of California, Davis, 6Department of Plant and Microbial Biology, University of California, Berkeley

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Protokollen er udviklet til effektivt at udtrække intakte histoner fra sorghum bladmaterialer til profilering af histone post-translationelle modifikationer, der kan tjene som potentielle epigenetiske markører til støtte engineering tørke resistente afgrøder.

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Biokemi tørke epigenetisk histone klipning post-translationelle ændringer proteomics sorghum top down massespektrometri
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