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Journal / Biochemistry / Isolering av Histone fra Sorghum Leaf Tissue for…
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Biochemistry

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JoVE Journal Biochemistry
Isolering av Histone fra Sorghum Leaf Tissue for Top Down Mass Spectrometry Profilering av potensielle epigenetiske markører
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Journal (Biochemistry)
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Author Spotlight: Illuminating New Avenues for Adipose Tissue Metabolism and Disease Prevention (Video) | JoVE
Journal (Biochemistry)
Author Spotlight: Illuminating New Avenues for Adipose Tissue Metabolism and Disease Prevention (Video) | JoVE
 
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Isolering av Histone fra Sorghum Leaf Tissue for Top Down Mass Spectrometry Profilering av potensielle epigenetiske markører

Article DOI: 10.3791/61707-v • 07:10 min • March 4th, 2021
March 4th, 2021 •
1, 1, 2, 2, 2, 1, 1, 1, 3, 3, 3, 4,5, 6, 6, 1, 6, 1, 1
1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute, Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center, University of California Agriculture and Natural Resources, 4West Side Research and Extension Center, University of California, 5Department of Plant Sciences, University of California, Davis, 6Department of Plant and Microbial Biology, University of California, Berkeley

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Protokollen er utviklet for effektivt å trekke ut intakte histoner fra sorghumbladmaterialer for profilering av histone post-translational modifikasjoner som kan tjene som potensielle epigenetiske markører for å hjelpe engineering tørkebestandige avlinger.

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Biokjemi Utgave 169 tørke epigenetisk histone klipping post-translasjonelle modifikasjoner proteomics sorghum topp ned massespektrometri
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