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Samtidig Live Imaging af flere insekt embryoner i Sample Kammer-baserede Light Sheet Fluorescens Mikroskoper
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Samtidig Live Imaging af flere insekt embryoner i Sample Kammer-baserede Light Sheet Fluorescens Mikroskoper

Article DOI: 10.3791/61713-v • 08:29 min • September 9th, 2020
September 9th, 2020 •
*1, *1, 1
1Physical Biology/Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt, Macromolecular Complexes (CEF - MC), Goethe-Universität
* These authors contributed equally

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Lysarkbaseret fluorescensmikroskopi er det mest værdifulde værktøj inden for udviklingsbiologi. Et vigtigt spørgsmål i sammenlignende undersøgelser er variansen i omgivelserne. Vores protokol beskriver en eksperimentel ramme for samtidig live billeddannelse af flere prøver og behandler derfor dette problem proaktivt.

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Biologi sammenlignende levende billeddannelse samtidig levende billeddannelse tre-dimensionelle lys mikroskopi lys ark-baseret fluorescens mikroskopi LSFM spindelvæv indehaveren insekt udvikling embryonale morfogenese embryogenese Drosophila melanogaster Tribolium castaneum omgivende varians
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