Biology
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Medición de distribuciones de cAMP tridimensionales en células vivas utilizando imágenes y análisis FRET hiperespectrales de 4 dimensiones (x, y, z y λ)
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Summary October 27th, 2020
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Debido a la baja relación señal-ruido (SNR) inherente de los sensores basados en transferencia de energía de resonancia Fӧrster (FRET), la medición de señales cAMP ha sido un desafío, especialmente en tres dimensiones espaciales. Aquí, describimos una metodología de imagen y análisis FRET hiperespectral que permite la medición de la distribución de cAMP en tres dimensiones espaciales.
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