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Messung von 3-dimensionalen cAMP-Verteilungen in lebenden Zellen mittels 4-dimensionaler (x, y, z und λ) hyperspektraler FRET-Bildgebung und -Analyse
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Messung von 3-dimensionalen cAMP-Verteilungen in lebenden Zellen mittels 4-dimensionaler (x, y, z und λ) hyperspektraler FRET-Bildgebung und -Analyse

Article DOI: 10.3791/61720-v • 08:22 min • October 27th, 2020
October 27th, 2020 •
1,2, 3, 1, 3, 1,2, 1,2, 1,2,3
1Department of Pharmacology, University of South Alabama, 2Center for Lung Biology, University of South Alabama, 3Department of Chemical and Biomolecular Engineering, University of South Alabama

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Aufgrund des inhärenten niedrigen Signal-Rausch-Verhältnisses (SNR) von Fӧrster-Resonanz-Energieübertragungssensoren (FRET) war die Messung von cAMP-Signalen eine Herausforderung, insbesondere in drei räumlichen Dimensionen. Hier beschreiben wir eine hyperspektrale FRET-Bildgebungs- und Analysemethodik, die die Messung der cAMP-Verteilung in drei räumlichen Dimensionen ermöglicht.

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Biologie Ausgabe 164 Zyklische AMP räumlich FRET hyperspektral Mikroskopie spektrale FRET
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