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Måling af 3-dimensionelle cAMP-fordelinger i levende celler ved hjælp af 4-dimensionelle (x, y, z og λ) Hyperspektral FRET Imaging and Analysis
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Måling af 3-dimensionelle cAMP-fordelinger i levende celler ved hjælp af 4-dimensionelle (x, y, z og λ) Hyperspektral FRET Imaging and Analysis

Article DOI: 10.3791/61720-v • 08:22 min • October 27th, 2020
October 27th, 2020 •
1,2, 3, 1, 3, 1,2, 1,2, 1,2,3
1Department of Pharmacology, University of South Alabama, 2Center for Lung Biology, University of South Alabama, 3Department of Chemical and Biomolecular Engineering, University of South Alabama

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På grund af iboende lave signal-til-støj-forhold (SNR) af Fӧrster resonans energioverførsel (FRET) baserede sensorer, måling af cAMP signaler har været udfordrende, især i tre rumlige dimensioner. Her beskriver vi en hyperspektral FRET billed- og analysemetode, der gør det muligt at måle cAMP-distribution i tre rumlige dimensioner.

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Biologi Udgave 164 Cyklisk AMP rumlig FRET hyperspektral mikroskopi spektral FRET
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