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Journal / Chemistry / Atomkraftmikroskopi kombinert med infrarød…
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Chemistry

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JoVE Journal Chemistry
Atomkraftmikroskopi kombinert med infrarød spektroskopi som et verktøy for å sondere enkeltbakteriekjemi
Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Journal (Chemistry)
Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
Journal (Chemistry)
Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
 
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Atomkraftmikroskopi kombinert med infrarød spektroskopi som et verktøy for å sondere enkeltbakteriekjemi

Article DOI: 10.3791/61728-v • 08:51 min • September 15th, 2020
September 15th, 2020 •
1, 2,3, 1,2, 1
1Centre for Biospectroscopy and School of Chemistry, Monash University, 2Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, 3Department of Infectious Diseases, The Alfred Hospital and Central Clinical School, Monash University

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Atomic Force Microscopy-Infrared Spectroscopy (AFM-IR) gir en kraftig plattform for bakteriestudier, noe som gjør det mulig å oppnå nanoskala oppløsning. Begge deler, kartlegging av subcellulære endringer (f.eks. ved celledeling) samt komparative studier av kjemisk sammensetning (f.eks. som følge av legemiddelresistens) kan utføres på et enkelt cellenivå i bakterier.

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Kjemi Utgave 163 AFM-IR bakterier antimikrobiell resistens AMR bakteriell cellevegg avbildning Stafylokokker aureus methicillin-resistens MRSA vancomycinresistens VISA daptomycin ikke-følsomhet
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