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Journal / Biology / Kryogen prøvebelastning i en magisk vinkel…
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Kryogen prøvebelastning i en magisk vinkel spinning nuklear magnetisk resonansspektrometer, der bevarer cellulær levedygtighed
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Kryogen prøvebelastning i en magisk vinkel spinning nuklear magnetisk resonansspektrometer, der bevarer cellulær levedygtighed

Article DOI: 10.3791/61733-v • 06:42 min • September 1st, 2020
September 1st, 2020 •
*1, *1, *1, 1,2
1Department of Biophysics, University of Texas Southwestern Medical Center, 2Center for Alzheimer's and Neurodegenerative Disease and Center for Systems Biology, University of Texas Southwestern Medical Center
* These authors contributed equally

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Præsenteret her er en protokol for kryogen overførsel af frosne prøver i den dynamiske nukleare polarisering (DNP) magiske vinkel spinning (MAS) nukleare magnetiske resonans (NMR) sonde. Protokollen indeholder anvisninger til rotorlagring forud for eksperimentet og anvisninger for levedygtighedsmålinger før og efter forsøget.

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Biologi Problem 163 kryopræservering DNP MAS NMR solid-state NMR in-cell NMR NMR spektroskopi
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