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Journal / Biology / Nicotiana benthamiana'da Geçici Olarak Ifade…
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Nicotiana benthamiana'da Geçici Olarak Ifade Edilen IgG Füzyon Proteinlerinin Üretimi
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Nicotiana benthamiana'da Geçici Olarak Ifade Edilen IgG Füzyon Proteinlerinin Üretimi

Article DOI: 10.3791/61774-v • 13:29 min • January 16th, 2021
January 16th, 2021 •
*1,2,3, *1,2,3, 1,2,3, 1,2,4, 2,3, 1,2,3, 1,2,3
1Center for Immunotherapy, Vaccines and Virotherapy, The Biodesign Institute, Arizona State University, 2School of Life Sciences, Arizona State University, 3Molecular Biosciences/Biotechnology Undergraduate Program, Arizona State University, 4Department of Microbiology and Immunology, University of Michigan Medical School
* These authors contributed equally

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Burada ifade, çıkarma ve rekombinant insan IgG arınma için basit bir yöntem Nicotiana benthamianaGFP erimiş açıklar. Bu protokol, kolon kromatografisi kullanan çok sayıda proteinin saflaştırılması ve görselleştirilmesi için genişletilebilir. Ayrıca protokol, proje tabanlı keşif ler sağlayan, şahsenn ve sanal üniversite öğretim laboratuvarına uyarlanabilir.

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Biyoloji Sayı 167 antikor monoklonal antikor moleküler pharming agroinfiltrasyon geçici ekspresyon Nicotiana benthamiana yeşil floresan protein asit kararlı yeşil floresan protein antikor füzyonu IgG-füzyon protein G arıtma
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