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JoVE Journal Neuroscience
Generazione di neuroni umani e oligodendrociti da cellule staminali pluripotenti per la modellazione delle interazioni neurone-oligodendrociti
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Generazione di neuroni umani e oligodendrociti da cellule staminali pluripotenti per la modellazione delle interazioni neurone-oligodendrociti

Article DOI: 10.3791/61778-v • 10:53 min • November 9th, 2020
November 9th, 2020 •
*1, *1,2, *3, 1, 1, 3, 3,4, 1,5,6
1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 2Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 5Department of Neurology, Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University
* These authors contributed equally

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Le interazioni neurone-glia nella neurodegenerazione non sono ben comprese a causa di strumenti e metodi inadeguati. Qui, descriviamo protocolli ottimizzati per ottenere neuroni indotti, cellule precursori degli oligodendrociti e oligodendrociti da cellule staminali pluripotenti umane e forniamo esempi dei valori di questi metodi nella comprensione dei contributi specifici del tipo di cellula nella malattia di Alzheimer.

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Neuroscienze Numero 165 cellule staminali pluripotenti indotte cellule iPS cellule staminali embrionali umane cellule ES neuroni oligodendrociti cellule progenitrici degli oligodendrociti OPC morbo di Alzheimer peptidi amiloide-beta sinapsi
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