JoVE Monthly Highlights: May 2018

1
1JoVE Content Production

Tickling, a Technique for Inducing Positive Affect When Handling Rats

Sylvie Cloutier1,2, Megan R. LaFollette3, Brianna N. Gaskill3, Jaak Panksepp1, Ruth C. Newberry4
1Center for the Study of Animal Well-being, Department of Integrative Physiology and Neuroscience, Washington State University, 2Canadian Council on Animal Care, 3Department of Animal Sciences, Center for Animal Welfare Science, College of Agriculture, Purdue University, 4Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences

This article demonstrates the standardized application of playful handling, a tickling technique designed to mimic rat rough-and-tumble play. This technique is effective at reducing fearful reactions to humans and generating positive affect when rats are handled for common husbandry activities and medical and research procedures such as injection.

Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

Amanda F. Bolgioni1, Marc A. Vittoria1, Neil J. Ganem1,2
1Department of Pharmacology & Experimental Therapeutics, Boston University School of Medicine, 2Department of Medicine, Section of Hematology and Oncology, Boston University School of Medicine

Live-cell imaging provides a wealth of information on single cells or whole populations that is unattainable by fixed cell imaging alone. Here, live-cell imaging protocols to assess cell fate decisions following treatment with the anti-mitotic drug paclitaxel are described.

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Behnom Farboud*1,2, Erin Jarvis*1, Theodore L. Roth*3,4,5,6, Jiyung Shin*1,3, Jacob E. Corn1,3, Alexander Marson3,5,6,7,8,9, Barbara J. Meyer1,2, Nipam H. Patel1,10, Megan L. Hochstrasser3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

Utilizing a preassembled Cas9 ribonucleoprotein complex (RNP) is a powerful method for precise, efficient genome editing. Here, we highlight its utility across a broad range of cells and organisms, including primary human cells and both classic and emerging model organisms.

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Robert W Epps1, Kobi C Felton1, Connor W Coley2, Milad Abolhasani1
1Chemical and Biomolecular Engineering, North Carolina State University, 2Chemical Engineering, Massachusetts Institute of Technology

Detailed herein are the operation and assembly protocols of a modular microfluidic screening platform for the systematic characterization of colloidal semiconductor nanocrystal syntheses. Through fully adjustable system arrangements, highly efficient spectra collection may be carried out across 4 orders of magnitude reaction time scales within a mass transfer-controlled sampling space.

PUBLISH WITH JoVE RECOMMEND JoVE