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Journal / Biochemistry / 通过质量光度法快速测定抗体抗原亲和力…
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Biochemistry

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JoVE Journal Biochemistry
通过质量光度法快速测定抗体抗原亲和力
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
Journal (Biochemistry)
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
Journal (Biochemistry)
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
 
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通过质量光度法快速测定抗体抗原亲和力

Article DOI: 10.3791/61784-v • 10:17 min • February 8th, 2021
February 8th, 2021 •
1, 1
1Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health

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我们使用质量光度测量 （MP） 来描述抗原-抗体亲和力测量的单分子方法。基于MP的协议是快速，准确，使用非常少量的材料，不需要蛋白质修改。

Tags

生物化学，第168期，质量光度测量，抗体亲和力，蛋白质-蛋白质相互作用，无标签，结合亲和力测量，多价蛋白相互作用
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