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JoVE Journal Biochemistry
Hurtig bestemmelse af antistof-antigen affinitet ved massefotometri
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Hurtig bestemmelse af antistof-antigen affinitet ved massefotometri

Article DOI: 10.3791/61784-v • 10:17 min • February 8th, 2021
February 8th, 2021 •
1, 1
1Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health

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Vi beskriver en enkelt-molekyle tilgang til antigen-antistof affinitet målinger ved hjælp af masse fotometri (MP). MP-baserede protokol er hurtig, præcis, bruger en meget lille mængde materiale, og kræver ikke protein modifikation.

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Biokemi massefotometri antistofaffæritet proteinproteininteraktioner etiketfri bindende affinitetsmålinger multivalente proteininteraktioner
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