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JoVE Journal Biochemistry
Snelle bepaling van antilichaam-antigeen affiniteit door massa fotometrie
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Snelle bepaling van antilichaam-antigeen affiniteit door massa fotometrie

Article DOI: 10.3791/61784-v • 10:17 min • February 8th, 2021
February 8th, 2021 •
1, 1
1Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health

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We beschrijven een single-molecule benadering van antigeen-antilichaam affiniteit metingen met behulp van massa fotometrie (MP). Het MP-gebaseerde protocol is snel, nauwkeurig, gebruikt een zeer kleine hoeveelheid materiaal en vereist geen eiwitmodificatie.

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Biochemie massafotometrie antilichaamaffiniteit eiwit-eiwitinteracties labelvrije bindende affiniteitsmetingen multivalente eiwitinteracties
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