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Journal / Immunology and Infection / Analisi del repertorio immunitario del recettore…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Analisi del repertorio immunitario del recettore delle cellule T e B utilizzando il sequenziamento di nuova generazione
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Analisi del repertorio immunitario del recettore delle cellule T e B utilizzando il sequenziamento di nuova generazione

Article DOI: 10.3791/61792-v • 08:59 min • January 12th, 2021
January 12th, 2021 •
1,2, 3,4, 1, 3,4, 1,2
1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, 2Sackler Faculty of Medicine, Tel Aviv University, 3Cancer Research Center, Sheba Medical Center, 4Molecular Hematology Laboratory, Sheba Medical Center

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L'attuale protocollo descrive un metodo per l'isolamento del DNA da campioni di sangue e biopsie intestinali, generazione di librerie PCR TCRβ e IGH per il sequenziamento di nuova generazione, le prestazioni di una corsa NGS e l'analisi dei dati di base.

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Questo mese in JoVE Numero 167 Sequenziamento di nuova generazione TCRβ IGH repertorio immunitario immunità adattiva cellule T cellule B
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