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Developmental Biology

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JoVE Journal Developmental Biology
Transillumination-Assisted Dissectie van specifieke stadia van de muis seminifereuze epitheelcyclus voor downstream immunostaininganalyses
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Transillumination-Assisted Dissectie van specifieke stadia van de muis seminifereuze epitheelcyclus voor downstream immunostaininganalyses

Article DOI: 10.3791/61800-v • 09:59 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1, 1, 3,4, 1,2, 3,4, 1, 1
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, 2Department of Pediatrics, Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia

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Dit protocol beschrijft transillumination-ondersteunde microdissectie van segmenten van volwassen muis seminifereuze tubuli die specifieke stadia van seminifereuze epitheelcyclus vertegenwoordigen, en celtypen daarin, en daaropvolgende immunostaining van squashpreparaten en intacte tubulussegmenten.

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Ontwikkelingsbiologie muis spermatogenese seminiferous epitheel stadium transillumination squashpreparaat whole-mount vlekken spermiogenese
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