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Journal / Developmental Biology / Transillumination-assisteret dissektion af…
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Developmental Biology

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JoVE Journal Developmental Biology
Transillumination-assisteret dissektion af specifikke stadier af musen Seminiferous Epitel Cycle for Downstream Immunostaining Analyser
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Transillumination-assisteret dissektion af specifikke stadier af musen Seminiferous Epitel Cycle for Downstream Immunostaining Analyser

Article DOI: 10.3791/61800-v • 09:59 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1, 1, 3,4, 1,2, 3,4, 1, 1
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, 2Department of Pediatrics, Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia

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Denne protokol beskriver transilluminationsassisteret mikrodissection af segmenter af voksne mus seminiferous tubules, der repræsenterer specifikke stadier af seminiferous epitelcyklus, og celletyper deri, og efterfølgende immunfarvning af squashpræparater og intakte tubule segmenter.

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Udviklingsbiologi Problem 164 mus spermatogenese seminiferous epitel scene transillumination squashpræparat helmontering farvning spermiogenese
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