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Journal / Bioengineering / Dyrking av en tredimensjonal rekonstruert…
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Bioengineering

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JoVE Journal Bioengineering
Dyrking av en tredimensjonal rekonstruert menneskelig epidermis i stor skala
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Dyrking av en tredimensjonal rekonstruert menneskelig epidermis i stor skala

Article DOI: 10.3791/61802-v • 08:49 min • May 28th, 2021
May 28th, 2021 •
1, 1,2, 3, 3,4, 1, 2
1Adolphe Merkle Institute, University of Fribourg, 2Dow Silicones Belgium SRL, 3Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 4Department of Animal Sciences, Plants for Human Health Institute, North Carolina State University

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Denne protokollen beskriver en enkel metode for å dyrke tredimensjonale rekonstituerte menneskelige epidermis på en reproduserbar og robust måte. I tillegg karakteriserer den strukturfunksjonsforholdet til den epidermale barrieremodellen. De biologiske responsene til den rekonstituerte menneskelige epidermis ved proinflammatoriske stimuli presenteres også.

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Bioengineering utgave 171 rekonstituert menneskelig epidermis humane epidermale ekvivalenter primære keratinocytter epidermis in vitro-studier.
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