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JoVE Journal Cancer Research
Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
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Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Article DOI: 10.3791/61803-v • 05:24 min • February 21st, 2021
February 21st, 2021 •
*1, *1, 2, 1, 3, 1, 1,2,3
1Department of Biomedicine, University Hospital Basel and University of Basel, 2Division for Hematology, University Hospital Basel and University of Basel, 3Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen
* These authors contributed equally

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Summary
Automatic Translation

We present two different staining protocols for NKG2D ligand (NKG2DL) detection in human primary acute myeloid leukemia (AML) samples. The first approach is based on a fusion protein, able to recognize all known and potentially yet unknown ligands, while the second protocol relies on the addition of multiple anti-NKG2DL antibodies.

Tags

Flow Cytometric Approaches NKG2D Ligand Surface Detection Stem Cells Bulk Subpopulations Acute Myeloid Leukemia Staining Method Leukemic Stem Cells Biotin NKG2D Fusion Protein PBS Concentration AML Cells Cryovial Liquid Nitrogen Storage Thawing Medium
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