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Journal / Cancer Research / To flowcytometriske tilnærminger til NKG2D Ligand …
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JoVE Journal Cancer Research
To flowcytometriske tilnærminger til NKG2D Ligand overflatedeteksjon for å skille stamceller fra bulk subpopulations i akutt myeloid leukemi
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Journal (Cancer Research)
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Journal (Cancer Research)
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To flowcytometriske tilnærminger til NKG2D Ligand overflatedeteksjon for å skille stamceller fra bulk subpopulations i akutt myeloid leukemi

Article DOI: 10.3791/61803-v • 05:24 min • February 21st, 2021
February 21st, 2021 •
*1, *1, 2, 1, 3, 1, 1,2,3
1Department of Biomedicine, University Hospital Basel and University of Basel, 2Division for Hematology, University Hospital Basel and University of Basel, 3Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen
* These authors contributed equally

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Vi presenterer to forskjellige fargeprotokoller for NKG2D ligand (NKG2DL) deteksjon i humane primære akutte myeloid leukemi (AML) prøver. Den første tilnærmingen er basert på et fusjonsprotein, i stand til å gjenkjenne alle kjente og potensielt ukjente ligander, mens den andre protokollen er avhengig av tilsetning av flere anti-NKG2DL antistoffer.

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Kreftforskning Utgave 168 NKG2DL CD34 AML LSC fusjonsprotein FACS
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