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Journal / Immunology and Infection / M1 和 M2 人类单细胞衍生细胞的极化和对 结核病…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
M1 和 M2 人类单细胞衍生细胞的极化和对 结核病 感染的流动细胞学分析
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M1 和 M2 人类单细胞衍生细胞的极化和对 结核病 感染的流动细胞学分析

Article DOI: 10.3791/61807-v • 10:43 min • September 18th, 2020
September 18th, 2020 •
1,2, 1, 1, 3, 1, 1,4, 1
1Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, 2Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, 3Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura, Karolinska Institutet, 4Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet

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该协议提供了一种方法来研究人类M1或M2极化巨噬细胞中的 分枝杆菌 感染，该方法基于外周血单核细胞与感染GFP标记的病毒株H37Rv的巨噬细胞的分化，并使用10色面板（包括选定的M1/M2标记的表达）用流动细胞学进行分析。

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本月在JoVE，第163期，宏噬菌体，人，极化，M1/M2，结 核分枝杆菌，感染，免疫反应，流细胞测量
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