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Journal / Immunology and Infection / Polarisatie van M1 en M2 humane monocyt-afgeleide …
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Polarisatie van M1 en M2 humane monocyt-afgeleide cellen en analyse met flowcytometrie bij mycobacterium tuberculosis-infectie
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Polarisatie van M1 en M2 humane monocyt-afgeleide cellen en analyse met flowcytometrie bij mycobacterium tuberculosis-infectie

Article DOI: 10.3791/61807-v • 10:43 min • September 18th, 2020
September 18th, 2020 •
1,2, 1, 1, 3, 1, 1,4, 1
1Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, 2Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, 3Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura, Karolinska Institutet, 4Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet

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Dit protocol biedt een methode om Mycobacterium tuberculosis infectie in menselijke M1- of M2-gepolariseerde macrofagen te bestuderen op basis van differentiatie van perifere bloed-monocyten naar macrofaag-achtige cellen die zijn geïnfecteerd met de GFP-gelabelde virulente stam H37Rv, en geanalyseerd met flowcytometrie met behulp van een 10-kleurenpaneel inclusief expressie van geselecteerde M1/M2 markers.

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Deze maand in JoVE macrofaag mens polarisatie M1/M2 Mycobacterium tuberculosis infectie immuunrespons flow cytometrie
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