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Journal / Immunology and Infection / Polarisering av M1 og M2 Human Monocyte-Avledede…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Polarisering av M1 og M2 Human Monocyte-Avledede celler og analyse med Flow Cytometry på Mycobacterium tuberkulose infeksjon
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
Journal (Immunology and Infection)
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Journal (Immunology and Infection)
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
 
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Polarisering av M1 og M2 Human Monocyte-Avledede celler og analyse med Flow Cytometry på Mycobacterium tuberkulose infeksjon

Article DOI: 10.3791/61807-v • 10:43 min • September 18th, 2020
September 18th, 2020 •
1,2, 1, 1, 3, 1, 1,4, 1
1Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, 2Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, 3Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura, Karolinska Institutet, 4Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet

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Denne protokollen gir en metode for å studere Mycobacterium tuberkuloseinfeksjon i humane M1- eller M2-polariserte makrofager basert på differensiering av perifere blod-monocytter til makrofaglignende celler som er infisert med den GFP-merkede virulente stammen H37Rv, og analysert med strømningscytometri ved hjelp av et 10-fargers panel inkludert uttrykk for utvalgte M1 / M2-markører.

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Denne måneden i JoVE Utgave 163 makrofag human polarisering M1/M2 Mycobacterium tuberkulose infeksjon immunrespons strømningscytometri
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