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JoVE Journal Bioengineering
Segmentering av vekst av endotelceller i 6-brønnsplater på en orbital shaker for mekanobiologiske studier
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Segmentering av vekst av endotelceller i 6-brønnsplater på en orbital shaker for mekanobiologiske studier

Article DOI: 10.3791/61817-v • 12:28 min • June 3rd, 2021
June 3rd, 2021 •
*1,2, *1, 1, 2,3,4, 1
1Department of Bioengineering, Imperial College London, 2Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), 3Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, 4Singapore Eye Research Institute, The Academia
* These authors contributed equally

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Denne protokollen beskriver en beleggmetode for å begrense endotelcellevekst til et bestemt område av en 6-brønns plate for skjærspenningsapplikasjon ved hjelp av orbital shaker-modellen.

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Tilbaketrekning utgave 172 segmenteringsvekst endotelial skjærspenning orbital shaker mekanobiologi lav størrelse multiveis strømning høy størrelse uniaxial strømning PDMS Pluronic F-127 tverrgående veggskjær stress
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