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Journal / Environment / 开发和测试针对物种的定量PCR检测环境DNA应用…
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开发和测试针对物种的定量PCR检测环境DNA应用
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开发和测试针对物种的定量PCR检测环境DNA应用

Article DOI: 10.3791/61825-v • 08:54 min • November 5th, 2020
November 5th, 2020 •
*1, *1, *1, *1
1Columbia Environmental Research Center, U.S. Geological Survey
* These authors contributed equally

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环境DNA检测需要严格的设计、测试、优化和验证，才能开始收集现场数据。在这里，我们提出一个协议，让用户完成设计特定物种、基于探针的 qPCR 检测和量化环境样本中目标物种 DNA 的每一步。

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环境科学，第165期，eDNA，qPCR，针对性检测，物种特定，引物设计，检测验证，特异性，灵敏度
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