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Journal / Cancer Research / Undersøgelse TGF-β Signalering og TGF-β-induceret …
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Cancer Research

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JoVE Journal Cancer Research
Undersøgelse TGF-β Signalering og TGF-β-induceret Epitel-til-mesenchymal Overgang i brystkræft og normale celler
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Undersøgelse TGF-β Signalering og TGF-β-induceret Epitel-til-mesenchymal Overgang i brystkræft og normale celler

Article DOI: 10.3791/61830-v • 06:54 min • October 27th, 2020
October 27th, 2020 •
1, 1, 1, 1, 1
1Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center

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Vi beskriver en systematisk arbejdsgang for at undersøge TGF-β signalering og TGF-β-induceret EMT ved at studere det protein- og genekspression, der er involveret i denne signalvej. Metoderne omfatter vestlig blotting, en luciferase reporter assay, qPCR, og immunofluorescens farvning.

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Cancer Research omdanne vækstfaktor-β epitel-til-mesenchymal overgang brystkræft vestlige blotting transskriptionel reporter immunofluorescens hæmmere adipogenesis
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