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Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Article DOI: 10.3791/61833-v • 11:49 min • August 28th, 2021
August 28th, 2021 •
*1, *2, 2
1Centre for Research in Nanotechnology and Science, Indian Institute of Technology Bombay, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay
* These authors contributed equally

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The protocol aims to introduce the use of a triple quadrupole mass spectrometer for Multiple Reaction Monitoring (MRM) of proteins from clinical samples. We have provided a systematic workflow starting from sample preparation to data analysis for clinical samples with all the necessary precautions to be taken.

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Quantitative Proteomics Workflow Multiple Reaction Monitoring Targeted Proteomic Approaches Protein Detection Protein Quantification Human Brain Tissue MRM Experiment Brain Tissue Preparation Lysis Buffer Tissue Homogenization Protein Assay Protein Estimation
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