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Immunology and Infection

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JoVE Journal Immunology and Infection
Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes
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Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes

Article DOI: 10.3791/61835-v • 09:42 min • October 22nd, 2020
October 22nd, 2020 •
*1, *1, 2, 2
1Laboratory of Neurogenetics and Behavior, The Rockefeller University, 2Department of Biological Sciences, Columbia University
* These authors contributed equally

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Summary
Automatic Translation

The goal of this protocol is to deliver animal-derived and artificial blood meals to Aedes aegypti mosquitoes through an artificial membrane feeder and precisely quantify the volume of meal ingested.

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Feeding Quantifying Animal-derived Blood Artificial Meals Aedes Aegypti Mosquitoes High Throughput Measurements Meal Volumes Individual Mosquitoes Adaptive Content Of The Meal Experimental Groups User-friendly Low Cost Pharmacological Manipulations Genetic Manipulations Blood Feeding Post-blood Feeding Behavior Organization Setup And Reagents Defibrinated Sheep Blood Fluorescence-based Quantification Meal Size Fluorescein Solution Reference Standard Curve Protein-free Saline Meals
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